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SERCA1 ATPase antibody [IIH11]

Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818
Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818
Anti-SERCA1 ATPase antibody [IIH11] used in Western Blot (WB). GTX22818
Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818
Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818
Anti-SERCA1 ATPase antibody [IIH11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22818

Cat. No. GTX22818

Host

Mouse

Clonality

Monoclonal

Clone Name

IIH11

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IHC-Fr, IHC

Reactivity

Human, Mouse, Rat, Rabbit, Dog, Guinea pig
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF 1:500
IHC-P Assay dependent
IHC-Fr 1:20-200
IHC Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Product Note

This antibody detects sarcoplasmic or endoplasmic reticulum calcium 1 (SERCA1) ATPase. It recognizes an epitope between amino acids 199-505 of rabbit skeletal muscle ATPase, a region that is not exposed in native sarcoplasmic reticulum.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Rabbit

Immunogen

Purified rabbit skeletal muscle sarcoplasmic reticulum.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384873

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 , ATP2A3 , SERCA1a

Cellular Localization

Endoplasmic reticulum membrane

Database

Research Area

DATA IMAGES

Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818

GTX22818 IHC-P Image

IHC-P analysis of mouse skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818

GTX22818 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX22818 SERCA1 ATPase antibody [IIH11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-SERCA1 ATPase antibody [IIH11] used in Western Blot (WB). GTX22818

GTX22818 WB Image

WB analysis of 25 ug of human skeletal muscle (lane 1), rat skeletal muscle (lane 2) and mouse skeletal muscle (lane 3) using GTX22818 SERCA1 ATPase antibody [IIH11].
Dilution : 1:2000

Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818

GTX22818 IHC-P Image

IHC-P analysis of mouse lymph node tissue using GTX22818 SERCA1 ATPase antibody [IIH11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-SERCA1 ATPase antibody [IIH11] used in IHC (Paraffin sections) (IHC-P). GTX22818

GTX22818 IHC-P Image

IHC-P analysis of dog skeletal muscle tissue using GTX22818 SERCA1 ATPase antibody [IIH11].

Anti-SERCA1 ATPase antibody [IIH11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX22818

GTX22818 ICC/IF Image

ICC/IF analysis of C6 cells using GTX22818 SERCA1 ATPase antibody [IIH11]. Green : Primary antibody
Blue : Nuclei
Red : Actinbr>Fixation : formaldehyde
Dilution : 1:200 overnight at 4ºC

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399