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SHANK1 antibody [C3], C-term

Anti-SHANK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107701
Anti-SHANK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107701
Anti-SHANK1 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX107701
Anti-SHANK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107701
Anti-SHANK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107701
Anti-SHANK1 antibody [C3], C-term used in Western Blot (WB). GTX107701
Anti-SHANK1 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX107701

Cat. No. GTX107701

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all SHANK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

225 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human SHANK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951901

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH3 and multiple ankyrin repeat domains 1 , SPANK-1 , SSTRIP , synamon

Background

Seems to be an adapter protein in the postsynaptic density (PSD) of excitatory synapses that interconnects receptors of the postsynaptic membrane including NMDA-type and metabotropic glutamate receptors via complexes with GKAP/PSD-95 and Homer, respectively, and the actin-based cytoskeleton. May play a role in the structural and functional organization of the dendritic spine and synaptic junction.

Database

Research Area

DATA IMAGES

Anti-SHANK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107701

GTX107701 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using SHANK1(GTX107701) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SHANK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107701

GTX107701 ICC/IF Image

SHANK1 antibody [C3], C-term detects SHANK1 protein at cell body and synaptic vesicles by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, a neuron cell marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SHANK1 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX107701

GTX107701 IHC-Fr Image

SHANK1 antibody [C3] detects SHANK1 protein on embryonic mouse brain by immunohistochemical analysis. Sample: Frozen section of embryonic mouse brain (mE18.5). Red: SHANK1 antibody [C3] (GTX107701) diluted at 1:250. Blue: DAPI

Anti-SHANK1 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107701

GTX107701 ICC/IF Image

SHANK1 antibody [C3], C-term detects SHANK1 protein at cytoplasm by immunofluorescent analysis.
Sample: SKNSH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SHANK1 antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX107701

GTX107701 IHC-P Image

SHANK1 antibody [C3], C-term detects SHANK1 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: SHANK1 protein stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SHANK1 antibody [C3], C-term used in Western Blot (WB). GTX107701

GTX107701 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SHANK1 antibody [C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX107701

GTX107701 IHC-Fr Image

SHANK1 antibody [C3], C-term detects SHANK1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SHANK1 stained by SHANK1 antibody [C3], C-term (GTX107701) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169