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1 .GTX48138-PRO Shh Recombinant Protein (Active)

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ECL kit RIPA Extraction Kit Protein Ladder

Shh Recombinant Protein (Active)


  • ApplicationsELISA, WB, Functional Assay
Cat No: GTX48138-PRO
Package Price Qty
25 μgUSD 319.00
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  • Application Information: Shh Recombinant Protein (Active)

Catalog Number GTX48138-PRO
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Product Name Shh Recombinant Protein (Active)
See all Shh products
Full Namesonic hedgehog
SynonymsHHG1, SHH, HPE3, SMMCI, HLP3
BackgroundMembers of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue.
SpeciesHuman
Region/SequenceIVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
Expression SystemE. coli
ApplicationsELISA, WB, Functional Assay
Application NoteBiological activity was determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL - 226) cells. The expected ED50 for this effect is 0.8 - 1.0 µg/ml.
ConjugationUnconjugated
Form SuppliedLyophilized powder
PurityGreater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per μg (1EU/μg).
ConcentrationBatch dependent (Please refer to the vial label for the specific concentration)
Storage InstructionThe lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Hypoxia
  • Cancer > Type of cancer > Thyroid
  • Cell Biology > Endocytosis
  • Disease Related > Parkinson Disease
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Shh Recombinant Protein (Active) validated data
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All products are for research use only—Not for use in diagnostic procedures.