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Catalog Number
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GTX48138-PRO
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0
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Product Name
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Shh Recombinant Protein (Active)
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| Full Name | sonic hedgehog |
| Synonyms | HHG1, SHH, HPE3, SMMCI, HLP3 |
| Product Description | Active Recombinant Human Shh protein |
| Background | Members of the Hedgehog (Hh) family are highly conserved proteins which are widely represented throughout the animal kingdom. The three known mammalian Hh proteins, Sonic (Shh), Desert (Dhh) and Indian (Ihh) are structurally related and share a high degree of amino-acid sequence identity (e.g., Shh and Ihh are 93% identical). The biologically active form of Hh molecules is obtained by autocatalytic cleavage of their precursor proteins and corresponds to approximately the N-terminal one half of the precursor molecule. Although Hh proteins have unique expression patterns and distinct biological roles within their respective regions of secretion, they use the same signaling pathway and can substitute for each other in experimental systems. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the natural occurring chemically modified Cys residue. |
| Target | SHH |
| Species | Human |
| Expression System | E. coli |
| Applications | ELISA, WB, Functional Assay |
| Application Note | Biological activity was determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL - 226) cells. The expected ED50 for this effect is 0.8 - 1.0 µg/ml. |
| Form Supplied | Lyophilized powder |
| Purification Note | Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
| Purity | Greater than 98% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
| Storage Instruction | The lyophilized SHH recombinant protein is stable for at least 2 years from date of receipt at -20ºC. Reconstituted SHH is stable for at least 3 months when stored in working aliquots with a carrier protein at -20ºC. As with any protein, exposing SHH reco |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |
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