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1 .GTX111075 SMAD2 antibody

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SMAD2 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 2 )
Cat No: GTX111075
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111075
  • Anti-SMAD2 antibody used in Western Blot (WB). GTX111075
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
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  • Datasheet
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    Reference (2)
  • Bio-info
  • Protocol & MSDS
  • Application Information: SMAD2 antibody

Catalog Number GTX111075
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( 0 )
Publication ( 2 )
Product Name SMAD2 antibody
See all SMAD2 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size52 kDa (note) IsoForm (3)
Positive Controls293T , A431 , Jurkat , Raji
Form SuppliedLiquid
Concentration0.55 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: SMAD2 antibody

Full NameSMAD family member 2
BackgroundThe protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
SynonymsJV18 antibody, JV18-1 antibody, MADH2 antibody, MADR2 antibody, MGC22139 antibody, MGC34440 antibody, hMAD-2 antibody, hSMAD2 antibody, SMAD2 antibody, mother against DPP homolog 2 antibody, "SMAD, mothers against DPP homolog 2 antibody", MAD homolog 2 antibody, mothers against decapentaplegic homolog 2 antibody, Sma- and Mad-related protein 2 antibody, SMAD family member 2 antibody
Cellular LocalizationCytoplasm , Nucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Zebrafish, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteZebrafish (97%), Xenopus laevis (99%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (99%)
ConjugationUnconjugated

  • Storage Conditions: SMAD2 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Pancreatic
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Signal Transduction > Signaling through enzyme-linked cell surface receptors > TGF-Beta
  • Stem Cell Development > Development > Endoderm development
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SMAD2 antibody validated data
  • Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111075
    • GTX111075 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD2(GTX111075) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-SMAD2 antibody used in Western Blot (WB). GTX111075
    • GTX111075 WB Image
      Sample (30 ug of whole cell lysate)
      A: Jurkat
      10% SDS PAGE
      GTX111075 diluted at 1:500
      Top
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
    • GTX111075 IHC-P Image
      SMAD2 antibody detects SMAD2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse liver.
      SMAD2 antibody (GTX111075) diluted at 1:500.
      Top
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
    • GTX111075 IHC-P Image
      SMAD2 antibody detects SMAD2 protein at cytoplasm in rat testis by immunohistochemical analysis.
      Sample: Paraffin-embedded rat testis.
      SMAD2 antibody (GTX111075) diluted at 1:500.
      Top
  • Anti-SMAD2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111075
    • GTX111075 ICC/IF Image
      Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using SMAD2(GTX111075) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
      Top
  • Anti-SMAD2 antibody used in Western Blot (WB). GTX111075
    • GTX111075 WB Image
      Sample (30 ug of whole cell lysate)
      A: Jurkat
      10% SDS PAGE
      GTX111075 diluted at 1:500
      Top
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
    • GTX111075 IHC-P Image
      SMAD2 antibody detects SMAD2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse liver.
      SMAD2 antibody (GTX111075) diluted at 1:500.
      Top
  • Anti-SMAD2 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX111075
    • GTX111075 IHC-P Image
      SMAD2 antibody detects SMAD2 protein at cytoplasm in rat testis by immunohistochemical analysis.
      Sample: Paraffin-embedded rat testis.
      SMAD2 antibody (GTX111075) diluted at 1:500.
      Top
Back to Top
  • Reference for SMAD2 antibody
Application References Application Reactivity
Thiyagarajan V et al. Antroquinonol Targets FAK-Signaling Pathway Suppressed Cell Migration, Invasion, and Tumor Growth of C6 Glioma. PLoS One 10 (10):e0141285 (2015). WB Rat
Chen G et al. SREBP-1 is a novel mediator of TGFβ1 signaling in mesangial cells. J Mol Cell Biol 6 (6):516-30 (2014). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensSMAD24087Q15796, Q53XR6601366NM_001003652NP_00100365252.32
Homo sapiensSMAD24087Q15796, Q53XR6601366NM_001135937NP_00112940948.96
Homo sapiensSMAD24087Q15796, Q53XR6601366NM_005901NP_00589252.32

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusSmad217126Q62432NP_00129799948.96
Mus musculusSmad217126Q62432NM_010754NP_03488452.28
Rattus norvegicusSmad229357O70436NM_019191NP_06206452.25
Mus musculusSmad217126Q62432NP_00123941052.28
Rattus norvegicusSmad229357O70436NP_00126437952.25
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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