|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||48 kDa (note)
|Positive Controls||Jurkat , Mouse brain , Rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||SMAD family member 3|
|Product Description||Rabbit Polyclonal antibody to SMAD3 (SMAD family member 3)|
|Background||The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq]|
|Synonyms||DKFZp586N0721 antibody, DKFZp686J10186 antibody, HSPC193 antibody, HsT17436 antibody, JV15-2 antibody, MADH3 antibody, MGC60396 antibody, SMAD3 antibody, hSMAD3 antibody, mad homolog JV15-2 antibody, MAD homolog 3 antibody, hMAD-3 antibody, MAD, mothers against decapentaplegic homolog 3 antibody, SMA- and MAD-related protein 3 antibody, mad protein homolog antibody, mothers against DPP homolog 3 antibody, mothers against decapentaplegic homolog 3 antibody, mad3 antibody, SMAD, mothers against DPP homolog 3 antibody, SMAD family member 3 antibody|
|Cellular Localization||Cytoplasm (By similarity) , Nucleus (By similarity) |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human SMAD3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Dog, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Xenopus laevis (95%), Dog (100%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (99%)|