|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||60 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||SMAD family member 4|
|Product Description||Rabbit Polyclonal antibody to SMAD4 (mothers against decapentaplegic homolog 4)|
|Background||This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]|
|Synonyms||DPC4 antibody, JIP antibody, MADH4 antibody, SMAD4 antibody, "mothers against decapentaplegic, Drosophila, homolog of, 4 antibody", deletion target in pancreatic carcinoma 4 antibody, MAD homolog 4 antibody, "SMAD, mothers against DPP homolog 4 antibody", mothers against decapentaplegic homolog 4 antibody, deleted in pancreatic carcinoma locus 4 antibody, SMAD family member 4 antibody|
|Cellular Localization||Cytoplasm , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)|