|Applications||IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||143 kDa (note)
|Positive Controls||293T , A431 , H1299 , Molt-4 , NIH-3T3 , JC , BCL-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||structural maintenance of chromosomes 1A|
|Product Description||Rabbit Polyclonal antibody to SMC1A (structural maintenance of chromosomes protein 1A)|
|Background||Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation. [provided by RefSeq]|
|Synonyms||CDLS2 antibody, DKFZp686L19178 antibody, DXS423E antibody, KIAA0178 antibody, MGC138332 antibody, SB1.8 antibody, SMC1 antibody, SMC1L1 antibody, SMC1alpha antibody, SMCB antibody, SMC1A antibody, SMC protein 1A antibody, SMC1 structural maintenance of chromosomes 1-like 1 antibody, SMC-1A antibody, "SMC1 (structural maintenance of chromosomes 1, yeast)-like 1 antibody", SMC-1-alpha antibody, structural maintenance of chromosomes protein 1A antibody, segregation of mitotic chromosomes 1 antibody, structural maintenance of chromosomes 1A antibody|
|Cellular Localization||Nucleus , Kinetochore |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human SMC1A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Rat (100%), Zebrafish (89%), Xenopus laevis (96%), Chicken (95%), Bovine (100%)|