| Catalog Number | GTX70268 |
| Product Name | HEC1 antibody [9G3.23]
see all 'HEC1' products |
| Full Name | NDC80 homolog, kinetochore complex component (S. cerevisiae) |
| Synonyms | | HEC | 10403 | 607272 | O14777 | KNTC2 | HEC1 | |
| Product Description | Mouse monoclonal antibody to human HEC1 |
| Background | Human HEC 1 is a coiled-coil protein that has been demonstrated to have a role in M-phase progression. It is essential for proper chromosome segregation. Its depletion results inthe impairment of chromosome congression and results in spindle checkpoint activation. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No | 9G3.23 |
| Subclass | IgG |
| Immunogen | Human HEC1 protein consisting of amino acids 56-642. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse, Pig, African green monkey |
| Applications | Flow cytometry/FACS, ICC/IF, Immunoprecipitation, Western blot. The usefulness of this product in other applications has not been determined. |
| Application Note | For IF: Use at a concentration of 4-10 µg/ml. For IP: Use at a concentration of 1-5 µg/ml. For WB: Use at a dilution of 1:250 - 1:100.For FACS:Assay dependent Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. |
| Positive Controls | HeLa WCE |
| Target | HEC1 |
| Predicted Target Size(KDa) | 80 (Note) |
| Cellular Localization | Nuclear |
| Form Supplied | Liquid |
| Concentration | 1 mg/ml |
| Purification | Protein G Affinity Purified |
| Purification Note | From ascitic fluid |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |