SMURF2 antibody [N1N2], N-term
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||86 kDa (note)
|Positive Controls||A431 , H1299 , HeLaS3 , Molt-4 , Raji , MDA-MB-231|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||SMAD specific E3 ubiquitin protein ligase 2|
|Product Description||Rabbit Polyclonal antibody to SMURF2 (SMAD specific E3 ubiquitin protein ligase 2)|
|Background||E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.|
|Synonyms||DKFZp686F0270 antibody, MGC138150 antibody, SMURF2 antibody, E3 ubiquitin ligase SMURF2 antibody, hSMURF2 antibody, SMAD ubiquitination regulatory factor 2 antibody, E3 ubiquitin-protein ligase SMURF2 antibody, SMAD-specific E3 ubiquitin-protein ligase 2 antibody, SMAD specific E3 ubiquitin protein ligase 2 antibody|
|Cellular Localization||Nucleus , Cytoplasm , Cell membrane , Membrane raft |
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SMURF2. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Zebrafish|
|Predict Reactivity Note||Mouse (100%), Rat (100%), Zebrafish (100%)|