SOX2 antibody [GT1876]
|Applications||FACS, IHC-Fr, IP, WB|
|IHC (Frozen sections)||1:100-1:1000*
IHC (Frozen sections) : The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||34 kDa (note)
|Positive Controls||NT2D1 , PC-3 , U87-MG , SK-N-SH , human ESC , zebrafish eye|
(Please refer to the vial label for the specific concentration)|
|Purification||Affinity purified by Protein G.|
|Full Name||SRY (sex determining region Y)-box 2|
|Product Description||Mouse Monoclonal antibody [GT1876] to SOX2 (SRY (sex determining region Y)-box 2)|
|Background||This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]|
|Synonyms||ANOP3 antibody, MCOPS3 antibody, MGC2413 antibody, SOX2 antibody, transcription factor SOX2 antibody, SRY-related HMG-box gene 2 antibody, transcription factor SOX-2 antibody, SRY (sex determining region Y)-box 2 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Zebrafish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Japanese medaka, Cat, Pig, Chicken, Sheep, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Japanese medaka (81%), Cat (100%), Pig (98%), Chicken (94%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%)|