|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||81 kDa (note)
|Positive Controls||THP-1 , *Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Sp1 transcription factor|
|Product Description||Rabbit Polyclonal antibody to SP1 (Sp1 transcription factor)|
|Background||Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter. Plays an essential role in the regulation of FE65 gene expression. |
|Synonyms||SP1 antibody, transcription factor Sp1 antibody, specificity protein 1 antibody, Sp1 transcription factor antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (99%), Rhesus Monkey (99%), Bovine (98%)|