SQSTM1 antibody [GT1478]

Knockout/Knockdown validated

Host / ClonalityMouse Monoclonal
Clone NameGT1478
ApplicationsFACS, ICC/IF, IHC-Fr, WB
Species reactivityHuman, Mouse
★★★★★ ( 4 ) ( 1 )

Cat No:

GTX629890

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Star Review (4)
Reference (1)
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Protocol & MSDS

Application Information: SQSTM1 antibody [GT1478]

Catalog Number

GTX629890

Product Name

SQSTM1 antibody [GT1478]

See all SQSTM1 products

Applications

FACS, ICC/IF, IHC-Fr, WB

Application Note

	Suggested dilution	Reference
Flow cytometry/FACS	1:50-1:200*
Immunocytochemistry/ Immunofluorescence	1:100-1:1000*	Y
IHC (Frozen sections)	1:100-1:1000*
Western blot	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Positive Controls

HepG2 , A549 , H1299 , HCT116 , Huh-7 (untreated) , Huh-7 (3 µM Thapsigargin treatment for 12 hr) , Huh-7 (3 µM Thapsigargin treatment for 24 hr) , HepG2 (3 µM Thapsigargin treatment for 12 hr) , HepG2 (3 µM Thapsigargin treatment for 24 hr) , *2C4 , *MEF , *MNT-1

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Affinity purified by Protein G.

Specifications: SQSTM1 antibody [GT1478]

Full Name	sequestosome 1
Product Description	Mouse Monoclonal antibody to SQSTM1
Background	This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]
Synonyms	p62 Antibody, A170 Antibody, OSIL Antibody, sequestosome 1 Antibody, p62B Antibody, p60 Antibody, ZIP3 Antibody, PDB3 Antibody
Cellular Localization	Cytoplasm , Late endosome , Nucleus
Host	Mouse
Clonality	Monoclonal
Clone Name	GT1478
Target	SQSTM1
Isotype	IgG2b
Antigen Species	Human
Species Reactivity	Human, Mouse
Conjugation	Unconjugated

Storage Conditions: SQSTM1 antibody [GT1478]

Storage Buffer	PBS
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Apoptosis > Anti-apoptosis Cancer > Apoptosis > Induction > Extracellular signals Cell Biology > Apoptosis > Anti-apoptosis Cell Biology > Apoptosis > Induction from extracellular signals More Hide

SQSTM1 antibody [GT1478] validated data

GTX629890 WB Image

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) Huh-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 WB Image

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 WB Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
C. 30 µg HCT116 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [GT1478] (GTX629890) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 IHC-Fr Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Top

GTX629890 FACS Image

SQSTM1 antibody [GT1478] (GTX629890) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [GT1478] (GTX629890) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Top

GTX629890 ICC/IF Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:500.
Blue: Hoechst 33342 staining.

Top

GTX629890 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 ICC/IF Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Top

GTX629890 WB Image

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) Huh-7 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 WB Image

SQSTM1 antibody detects SQSTM1 protein by western blot analysis. Un-treated (-) and treated (+, Thapsigargin treatment for 12 hr and 24hr) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody (GTX629890) diluted by 1:1000.
The ACTB was used as internal control (GTX110564, 1:50000) shown at the bottom panel.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 WB Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein by western blot analysis.
A. 30 µg A549 whole cell lysate/extract
B. 30 µg H1299 whole cell lysate/extract
C. 30 µg HCT116 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [GT1478] (GTX629890) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 IHC-Fr Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Top

GTX629890 FACS Image

SQSTM1 antibody [GT1478] (GTX629890) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4ºC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [GT1478] (GTX629890) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Top

GTX629890 ICC/IF Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 16 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:500.
Blue: Hoechst 33342 staining.

Top

GTX629890 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Top

GTX629890 ICC/IF Image

SQSTM1 antibody [GT1478] detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [GT1478] (GTX629890) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, stained by phalloidin (invitrogen, A12380) diluted at 1:200.
Blue: Hoechst 33342 staining.

Top

StarReview
SQSTM1 antibody [GT1478]
Rating : ★★★★★ ( Average 5.0 based on 4 users reviews)
Western blot(4) :★★★★☆

Anonymous StarReview submitted on 17 Mar 2015
Score : ★★★★★
Application Tested : WB
Sample Species : Human
Sample : 2C4 cells
Amount used(ug/cells) : 50ug
Blocking conditions
Blocking Buffer : 5% non-fat milk
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/14hr

Anonymous StarReview submitted on 24 Apr 2015
Score : ★★★★★
Application Tested : WB
Sample Species : Human
Sample : MNT-1 cells
Amount used(ug/cells) : 30ug
Blocking conditions
Blocking Buffer : 5% skim milk in TBST
Incubation Time & Temperature : 25 (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/16hr

Anonymous StarReview submitted on 12 May 2016
Score : ★★★☆☆
Application Tested : WB
Sample Species : Human
Sample : HepG2 cells
Amount used(ug/cells) : 30ug
Blocking conditions
Blocking Buffer : 3% BSA in PBST
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 25 (℃)/overnight

Anonymous StarReview submitted on 18 Mar 2015
Score : ★★★★★
Application Tested : WB
Sample Species : Mouse
Sample : MEF cells
Amount used(ug/cells) : 50ug
Blocking conditions
Blocking Buffer : 5% non-fat milk
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:1000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/14hr

Reference for SQSTM1 antibody [GT1478]

Application References	Application	Reactivity
Gjyshi O et al. Kaposi's sarcoma-associated herpesvirus induces Nrf2 activation in latently infected endothelial cells through SQSTM1 phosphorylation and interaction with polyubiquitinated Keap1. J Virol 89 (4):2268-86 (2015).	ICC/IF	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	SQSTM1	8878	Q13501	601530, 602080	NM_001142298	NP_001135770	38.63

*MSDS

MSDS-PBS.pdf

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