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SQSTM1 / P62 antibody [N3C1], Internal

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Flow cytometry (FACS). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685
Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

Cat. No. GTX100685

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, PLA

Reactivity

Human, Mouse, Rat, Zebrafish, Bovine, Honeybee, Mosquito
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

SQSTM1 antibody, also known as ubiquitin-binding protein p62 antibody, detects the SQSTM1 protein that interacts with LC3 proteins to execute autophagosomal degradation. SQSTM1 is also reported to regulate the activation of NFκB1 downstream signaling in addition to having other cellular functions.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

48 kDa. ( Note )

Positive Control

NIH-3T3 , JC , BCL-1 , PC-12 , Rat2 , Huh7 ( 3μM Thapsigargin 12hr) , HepG2

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.39 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038029

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

Cellular Localization

Cytoplasm , Late endosome , Nucleus

Background

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685

GTX100685 ICC/IF Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Untreated (–) and treated (+) Huh-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein by western blot analysis.
A. 30 μg NIH-3T3 whole cell lysate/extract
B. 30 μg JC whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
12% SDS-PAGE
SQSTM1 antibody [N3C1], Internal (GTX100685) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100685

GTX100685 IHC-P Image

SQSTM1 / P62 antibody [N3C1], Internal detects SQSTM1 / P62 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunoprecipitation (IP). GTX100685

GTX100685 IP Image

Immunoprecipitation of SQSTM1 protein from HeLa whole cell extracts using 5 μg of SQSTM1 antibody [N3C1], Internal (GTX100685).
Western blot analysis was performed using SQSTM1 antibody [N3C1], Internal (GTX100685).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Flow cytometry (FACS). GTX100685

GTX100685 FACS Image

SQSTM1 antibody [N3C1], Internal (GTX100685) detects SQSTM1 protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 antibody [N3C1], Internal] (GTX100685) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100685

GTX100685 ICC/IF Image

SQSTM1 antibody [N3C1], Internal detects SQSTM1 protein at autophagosome by immunofluorescent analysis.
Samples: HepG2 cells treated with 3μM thapsigargin 12 hrs (rigtht) and mock (left) were fixed in ice-cold MeOH for 10 min, permeabilize with cooled acetone for 1 min .
Green: SQSTM1 protein stained by SQSTM1 antibody [N3C1], Internal (GTX100685) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SQSTM1 / P62 antibody [N3C1], Internal used in Western Blot (WB). GTX100685

GTX100685 WB Image

Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [N3C1], Internal (GTX100685) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

SQSTM1 / P62 antibody [N3C1], Internal Cat. No. GTX100685

Rating ( Average 4.8 based on 13 users reviews)
Application
Western Blot(WB) ( Average 4.8 based on 12 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 03-Oct-2022
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the 2020 in Int J Mol Sci. PMID: 33260618
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS WT and SQSTM1 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 08-Mar-2022
Score :
Application Tested : IHC-P
Sample Species : Rat
Sample : Rat eye tissue section
Blocking : 5% goat serum, RT, 1Hr
Fixation : PFA
Permeabilization /Antigen retrieval : TBS-T / None
Primary Antibody : 1:100, 4°C, 12Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Front Pharmacol in 2017.PMID: 29326587
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Antioxidants (Basel) in 2019.PMID: 31906147
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Antioxidants (Basel) in 2019.PMID: 31906147
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31505885
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31505885
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Biomolecules in 2019.PMID: 31505885
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2015.PMID: 25946033
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2017. PMID: 28134285
Date : Anonymous submitted on 17-Mar-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 50μg
Blocking : 5% non-fat milk, 4°C, 14Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Date : Anonymous submitted on 26-Jan-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Zheng ZY et al. Sci Rep 2018; 8 (1):16932
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169