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  • SSEA-5 antibody [8e11]

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1 .GTX70019 SSEA-5 antibody [8e11]

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SSEA-5 antibody [8e11]


  • Host / ClonalityMouse Monoclonal
  • Clone Name8e11
  • ApplicationsFACS, ICC/IF, IHC
  • Species reactivityHuman
  • Publication  ( 2 )
Cat No: GTX70019
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-SSEA-5 antibody [8e11] used in Flow cytometry/FACS. GTX70019
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
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  • Protocol & MSDS
  • Application Information: SSEA-5 antibody [8e11]

Catalog Number GTX70019
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Publication ( 2 )
Product Name SSEA-5 antibody [8e11]
See all SSEA-5 products
ApplicationsFACS, ICC/IF, IHC
Application NoteSuggested starting dilutions are as follows: ICC/IF: 1:50-1:1000, Flow: 1:50. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationProtein G affinity purified

  • Specifications: SSEA-5 antibody [8e11]

Full NameStage-Specific Embryonic Antigen-5
BackgroundStage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures.
SynonymsStage-Specific Embryonic Antigen-5, SSEA-5
Cellular LocalizationCell membrane
SpecificitySSEA-5
HostMouse
ClonalityMonoclonal
Clone Name8e11
IsotypeIgG
ImmunogenUndifferentiated human embryonic stem cells
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: SSEA-5 antibody [8e11]

Storage BufferPBS
Storage InstructionStore at -20 °C. Avoid repeated freeze/thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
SSEA-5 antibody [8e11] validated data
  • Anti-SSEA-5 antibody [8e11] used in Flow cytometry/FACS. GTX70019
    • GTX70019 FACS Image
      Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.
      Top
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
    • GTX70019 ICC/IF Image
      Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution).
      (A) Brightfield image
      (B) Dapi
      (C) anti-SSEA-5 Ab
      (D) Merged image of anti-SSEA-5 Ab and DAPI
      Top
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
    • GTX70019 ICC/IF Image
      Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.
      Top
  • Anti-SSEA-5 antibody [8e11] used in Flow cytometry/FACS. GTX70019
    • GTX70019 FACS Image
      Pluripotent stem cell population indicated by SSEA5-staining. Human embryonic stem cells H9 were analyzed with SSEA-5 mAB (pseudo-colored in red) and control IgG (pseudo-colored in green) in flow cytometry. Each Ab was used at a 1:50 dilution in PBS.
      Top
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
    • GTX70019 ICC/IF Image
      Immunofluorescent analysis of 4% FA fixed human embryonic stem cells H9 using anti-SSEA-5 Ab (1:50 dilution).
      (A) Brightfield image
      (B) Dapi
      (C) anti-SSEA-5 Ab
      (D) Merged image of anti-SSEA-5 Ab and DAPI
      Top
  • Anti-SSEA-5 antibody [8e11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70019
    • GTX70019 ICC/IF Image
      Immunofluorescent analysis of 4% PFA fixed human embryonal carcinoma cell line Tera using anti-SSEA-5 Ab (1:1000 dilution). SSEA-5 positive cells (red) represented pluripotent population; negative cells represented differentiated population. DAPI (Blue) indicated nuclear staining.
      Top
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  • Reference for SSEA-5 antibody [8e11]
Application References Application Reactivity
Cassidy L et al. Immunoreactivity of Pluripotent Markers SSEA-5 and L1CAM in Human Tumors, Teratomas, and Induced Pluripotent Stem Cells. J Biomark 2013:960862 (2013). ICC/IF Human
Clone References Application Reactivity
Tang C et al. An antibody against SSEA-5 glycan on human pluripotent stem cells enables removal of teratoma-forming cells. Nat Biotechnol 29 (9):829-34 (2011). IHC Human
    *MSDS
    MSDS-PBS.pdf
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