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Catalog Number
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GTX70019
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0
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Product Name
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SSEA-5 antibody [8e11]
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| Full Name | Stage-Specific Embryonic Antigen-5 |
| Synonyms | Stage-Specific Embryonic Antigen-5, SSEA-5 |
| Product Description | Mouse monoclonal antibody to SSEA-5 [8e11] |
| Specificity | SSEA-5 |
| Background | Stage-specific embryonic antigen (SSEA)-5 is an H type-1 glycan that is highly and specifically expressed on human embryonic stem cells (hESCs), the human blastocyst inner cell mass and undifferentiated human pluripotent stem cells (hPSCs). Expression of SSEA-5 in hESCs and hPSCs rapidly decreases upon differentiation and is considered an indicator of undifferentiated PSCs. This SSEA-5 antibody is capable of identifying undifferentiated pluripotent stem cells in heterogeneous populations, and when used in conjunction with other pluripotency surface markers (CD9 and CD90 or CD50 and CD200), it can be used to immunodeplete residual teratoma-causing cells from incompletely differentiated embryonic stem cell cultures. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | 8e11 |
| Isotype | IgG |
| Target | SSEA-5 |
| Immunogen | Undifferentiated human embryonic stem cells |
| Antigen Species | Human |
| Species Reactivity | Human |
| Applications | FACS, ICC/IF, IHC |
| Application Note |
| Suggested dilution | Protocol | Reference |
| Flow cytometry/FACS | * |
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| ICC/IF | 1:50-1:1000* |
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| Immunohistochemistry | * |
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Y |
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Cellular Localization | Cell membrane |
| Form Supplied | Liquid |
| Purification | Protein G affinity purified |
| Concentration | 1
mg/ml
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| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing no preservatives |
| Storage Instruction | Store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Notes | For in vitro research use only. Not for any diagnostic or therapeutic purpose. |