GeneTex

  • Country / Location Selection

United States (US)

STAT3 antibody [C3], C-term

KO/KD Comparison Orthogonal
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

Cat. No. GTX104616

Reference 30
4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Plant
Package
100 μl ($399),
25 μl ($169)
See all STAT3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

Positive Control

Human ESC , OC3

Predict Reactivity

Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAT3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952032

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

Cellular Localization

Cytoplasm , Nucleus

Background

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

KO/KD

Non-transfected (–) and transfected (+) OC3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

KO/KD

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-STAT3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104616

GTX104616 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3(GTX104616) antibody at 1:200 dilution.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Sample (20 μg of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX104616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29794990

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29794990

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2022 in Cell Death Dis. PMID: 35296653

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2022 in Cell Death Dis. PMID: 35296653

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2020 in PLoS One. PMID: 33382817

REFERENCE

Application Reference

Xu W et al. Immune Netw 2023; 23 (6) : e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis.
W Xu et al. Immune Netw 2023; 23 (6) : e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis
Application : WB
BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Liao CP et al. Biomedicines 2023; 11 (10) Methoxyhispolon Methyl Ether, a Hispolon Analog, Thwarts the SRC/STAT3/BCL-2 Axis to Provoke Human Triple-Negative Breast Cancer Cell Apoptosis In Vitro.
Hsieh YC et al. Biomedicines 2023; 11 (9) Blockade of the SRC/STAT3/BCL-2 Signaling Axis Sustains the Cytotoxicity in Human Colorectal Cancer Cell Lines Induced by Dehydroxyhispolon Methyl Ether.
Show More +
Submit a Reference

REVIEW

STAT3 antibody [C3], C-term Cat. No. GTX104616

Rating
( Average 4.3 based on 4 users reviews)
Application
Western Blot(WB)
( Average 4.3 based on 4 users reviews)
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 01-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : CH27 cells
Amount used : 20μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 28-Oct-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : T24 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in PBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : LAMA-84 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : TBST with 0.5% BSA, 4°C, 18Hr
Primary Antibody : 1:1000, 24°C, 2Hr
Submit a Review
Application Reference
Xu W et al. Immune Netw 2023; 23 (6):e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis.
W Xu et al. Immune Netw 2023; 23 (6):e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis
Application : WB
BY Wang et al. Am J Cancer Res 2023; 13 (10):4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Liao CP et al. Biomedicines 2023; 11 (10) Methoxyhispolon Methyl Ether, a Hispolon Analog, Thwarts the SRC/STAT3/BCL-2 Axis to Provoke Human Triple-Negative Breast Cancer Cell Apoptosis In Vitro.
Hsieh YC et al. Biomedicines 2023; 11 (9) Blockade of the SRC/STAT3/BCL-2 Signaling Axis Sustains the Cytotoxicity in Human Colorectal Cancer Cell Lines Induced by Dehydroxyhispolon Methyl Ether.
Hsu CY et al. Int J Mol Sci 2023; 24 (14) Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Kuo MY et al. Int J Mol Sci 2022; 24 (1) Hispolon Methyl Ether, a Hispolon Analog, Suppresses the SRC/STAT3/Survivin Signaling Axis to Induce Cytotoxicity in Human Urinary Bladder Transitional Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Mang Y et al. Ann Transl Med 2022; 10 (19):1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
Application : WB
Reactivity : Rat
Vidhya Tangeda et al. Cell Death Dis 2022; 13 (3):241
Application : WB
Reactivity : Human
Lecheng Zhang et al. Cell Cycle 2021; 20 (11):1052-1066 Knockdown of hsa_circ_0134111 alleviates the symptom of osteoarthritis via sponging microRNA-224-5p.
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
Application : WB
Reactivity : Mouse
Teo WH et al. PLoS One 2020; 15 (12):e0244791 Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.
Application : WB
Reactivity : Mouse
Ogawa T et al. Am J Pathol 2020; 190 (3):577-585 Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) Regulates Epidermal Keratinization under Psoriatic Skin Inflammation.
Application : IHC-P
Reactivity : Mouse
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
Application : WB
Reactivity : Human
Yang Y et al. Cancer Cell Int 2020; 20:9 Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.
Application : WB
Reactivity : Human
Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.
Fan JJ et al. Antioxidants (Basel) 2019; 8 (11) Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.
Application : WB
Reactivity : Human
Qin F et al. J Enzyme Inhib Med Chem 2019; 34 (1):909-926 Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.
Application : WB
Reactivity : Human
Shen M et al. J Exp Clin Cancer Res 2019; 38 (1):149
Application : WB
Reactivity : Human
Chen PC et al. Int J Mol Sci 2018; 19 (6) anti-metastatic effects of antrodan with and without * on lewis lung carcinomas in a mouse xenograft model.
Application : WB
Reactivity : Mouse
Lin C.I. et al. Int. J. Biol. Sci. 2018; 14 (3):253-265 Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice.
Application : WB
Reactivity : Mouse
Lin JT et al. Food Funct. 2017; 8 (3):1245-1253 Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.
Application : WB
Reactivity : Plant
Li X et al. Biomed Pharmacother 2017; 96:434-442 Targeting of cell cycle and let-7a/STAT3 pathway by niclosamide inhibits proliferation, migration and invasion in oral squamous cell carcinoma cells.
Application : WB
Reactivity : Human
Aalinkeel R et al. J Neuroimmune Pharmacol 2016; (Epub) Galectin-1 Reduces Neuroinflammation via Modulation of Nitric Oxide-Arginase Signaling in HIV-1 Transfected Microglia: a Gold Nanoparticle-Galectin-1 "Nanoplex" a Possible Neurotherapeutic?
Application : ICC/IF
Reactivity : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
Application : WB
Reactivity : Human
Huang JY et al. Sci Rep 2016; 6:34740 MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms.
Application : WB
Reactivity : Human
Hung AC et al. Clin Cancer Res 2016; (Epub) Extracellular Visfatin-Promoted Malignant Behavior in Breast Cancer Is Mediated Through c-Abl and STAT3 Activation.
Application : WB
Reactivity : Human
Chen YT et al. Oncotarget 2015; 6 (15) ptprd silencing by dna hypermethylation decreases * receptor signaling and leads to type 2 diabetes.
Application : WB
Reactivity : Human
Wang SH et al. Gastroenterology 2012; 142 (4):989-998.e4 Estrogen receptor α represses transcription of HBV genes via interaction with hepatocyte nuclear factor 4α.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169