|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoprecipitation||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||63 kDa (note)
|Positive Controls||HepG2 , Molt-4 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||stress-induced-phosphoprotein 1|
|Product Description||Rabbit Polyclonal antibody to STIP1 (stress-induced-phosphoprotein 1)|
|Background||STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM]|
|Synonyms||HOP antibody, IEF-SSP-3521 antibody, P60 antibody, STI1 antibody, STI1L antibody, STIP1 antibody, hsc70/Hsp90-organizing protein antibody, renal carcinoma antigen NY-REN-11 antibody, Hsp70/Hsp90-organizing protein antibody, NY-REN-11 antigen antibody, transformation-sensitive protein IEF SSP 3521 antibody, stress-induced-phosphoprotein 1 antibody|
|Cellular Localization||Cytoplasm (By similarity) , Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human STIP1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Chimpanzee, Bovine|
|Predict Reactivity Note||Xenopus laevis (84%), Chimpanzee (100%), Bovine (96%)|