|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||69 kDa (note)
|Positive Controls||Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||SWAP switching B-cell complex 70kDa subunit|
|Product Description||Rabbit Polyclonal antibody to SWAP70 (SWAP switching B-cell complex 70kDa subunit)|
|Background||Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. |
|Synonyms||SWAP-70 Antibody, HSPC321 Antibody, SWAP switching B-cell complex 70kDa subunit Antibody|
|Cellular Localization||Cytoplasm , Cell membrane , Nucleus , Cell projection , lamellipodium |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human SWAP70. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (95%), Chicken (89%), Rhesus Monkey (99%), Bovine (93%)|