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Sec61 gamma subunit antibody

Anti-Sec61 gamma subunit antibody used in Western Blot (WB). GTX101782
Anti-Sec61 gamma subunit antibody used in IHC (Whole mount) (IHC-Wm). GTX101782
Anti-Sec61 gamma subunit antibody used in Western Blot (WB). GTX101782

Cat. No. GTX101782

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Human, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all Sec61 gamma subunit products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

8 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Bovine, Dog, Chicken, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Sec61 gamma subunit. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1241303

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Sec61 translocon gamma subunit , SSS1

Cellular Localization

Endoplasmic reticulum membrane; Single-pass membrane protein

Background

The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. Oligomers of the Sec61 complex form a transmembrane channel where proteins are translocated across and integrated into the ER membrane. This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the gamma-subunit protein. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Sec61 gamma subunit antibody used in Western Blot (WB). GTX101782

GTX101782 WB Image

Zebrafish tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Sec61 gamma subunit antibody (GTX101782) diluted at 1:500.

Anti-Sec61 gamma subunit antibody used in IHC (Whole mount) (IHC-Wm). GTX101782

GTX101782 IHC-Wm Image

Immunohistochemical analysis (whole mount) of zebrafish embryo, using Sec61 gamma subunit antibody (GTX101782) at 1:200 dilution.

Anti-Sec61 gamma subunit antibody used in Western Blot (WB). GTX101782

GTX101782 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
12% SDS PAGE
GTX101782 diluted at 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169