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Separase antibody [XJ11-4D7]

Anti-Separase antibody [XJ11-4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15955

Cat. No. GTX15955

Host

Mouse

Clonality

Monoclonal

Clone Name

XJ11-4D7

Isotype

IgG2a

Application

ICC/IF

Reactivity

Human
Package
100 μl ($419)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:1000
Not tested in other applications.

Calculated MW

233 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Maltose-Binding Protein fusion of C-terminal fragment of human Separase (amino acids 1866-1996). [UniProt# Q14674]

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

extra spindle pole bodies like 1, separase , ESP1 , SEPA

Cellular Localization

Cytoplasm,Nucleus

Background

Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Separase antibody [XJ11-4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15955

GTX15955 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15955 Separase antibody [XJ11-4D7].
Red : primary antibody
Green : gamma tubulin
Blue : DAPI

REFERENCE

There are currently no references for Separase antibody [XJ11-4D7] (GTX15955). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Separase antibody [XJ11-4D7] (GTX15955). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf
Package List Price ($)
$ 419