Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||63 kDa (note)
|Positive Controls||293T , A431 , H1299 , HepG2 , Molt-4 , Raji , Neuro2A , 293 (input) , Stau1-IP (GTX106566) , Post-IP lysate from control rabbit IgG-IP , Post-IP lysate from Stau1-IP|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||staufen, RNA binding protein, homolog 1 (Drosophila)|
|Product Description||Rabbit Polyclonal antibody to Staufen (staufen, RNA binding protein, homolog 1 (Drosophila))|
|Background||Staufen is a member of the family of double-stranded RNA (dsRNA)-binding proteins involved in the transport and/or localization of mRNAs to different subcellular compartments and/or organelles. These proteins are characterized by the presence of multiple dsRNA-binding domains which are required to bind RNAs having double-stranded secondary structures. The human homologue of staufen encoded by STAU, in addition contains a microtubule- binding domain similar to that of microtubule-associated protein 1B, and binds tubulin. The STAU gene product has been shown to be present in the cytoplasm in association with the rough endoplasmic reticulum (RER), implicating this protein in the transport of mRNA via the microtubule network to the RER, the site of translation. Five transcript variants resulting from alternative splicing of STAU gene and encoding three isoforms have been described. Three of these variants encode the same isoform, however, differ in their 5'UTR. [provided by RefSeq]|
|Synonyms||FLJ25010 antibody, STAU antibody, STAU1 antibody, double-stranded RNA-binding protein Staufen homolog 1 antibody, "staufen, RNA binding protein, homolog 1 (Drosophila) antibody"|
|Cellular Localization||Rough endoplasmic reticulum|
|Specificity||Knockdown/Knockout validation was supported by references (PMID:26990999)|
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human Staufen. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey|
|Predict Reactivity Note||Rat (94%), Zebrafish (81%), Xenopus laevis (86%), Chicken (94%), Rhesus Monkey (100%)|