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1 .GTX85912 Streptavidin (HRP)

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Streptavidin (HRP)


  • ApplicationsELISA, IHC, WB
  • Publication  ( 1 )
Cat No: GTX85912
Package Price Qty
500 μgUSD 99.00
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  • Application Information: Streptavidin (HRP)

Catalog Number GTX85912
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Publication ( 1 )
Product Name Streptavidin (HRP)
See all Streptavidin products
Product DescriptionPeroxidase conjugated Streptavidin
BackgroundStreptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed.
ApplicationsELISA, IHC, WB
Application NoteELISA: 1:10,000-1:50,000 (20-100 ng/ml); IHC: 1:200-1:1:1,000 (1-5 µg/ml); WB (ECL) 1:10,000-1:100,000 (10-100 ng/ml); WB (chromogen) 1:500-1:5,000 (0.1-2 µg/ml). The optimum dilution should be determined by the individual investigator.
ConjugationHRP
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
Storage Buffer10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300
Storage Instruction2-8°C
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Streptavidin (HRP) validated data
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  • Reference for Streptavidin (HRP)
Application References Application Reactivity
Kung YA et al. Control of the negative IRES trans-acting factor KHSRP by ubiquitination. Nucleic Acids Res (2016). WB
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All products are for research use only—Not for use in diagnostic procedures.