|
Catalog Number
|
GTX85912
(
1
)
|
|
Product Name
|
Streptavidin (HRP)
|
| Product Description | Peroxidase conjugated Streptavidin |
| Background | Streptavidin is a 22- 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Peroxidase used for conjugation is 44 kDa glycoprotein isolated from horseradish roots. The Rz (Reinhetszahl), absorbance ratio of A403/A275 is ~3.0. One mg of Streptavidin is conjugated with peroxidase, in equimolar ratio. Unconjugated Streptavidin is removed. |
| Applications | ELISA, IHC, WB |
| Application Note | ELISA: 1:10,000-1:50,000 (20-100 ng/ml); IHC: 1:200-1:1:1,000 (1-5 µg/ml); WB (ECL) 1:10,000-1:100,000 (10-100 ng/ml); WB (chromogen) 1:500-1:5,000 (0.1-2 µg/ml). The optimum dilution should be determined by the individual investigator. |
| Conjugation | HRP |
| Form Supplied | Liquid |
| Concentration | 1
mg/ml
(Please refer to the vial label for the specific concentration) |
| Storage Buffer | 10 mM PO4, 200 mM NaCl, pH 7.5, BSA 10 mg/ml and 0.05% Proclin 300 |
| Storage Instruction | 2-8°C |
| Notes | For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption. |