Syndecan-1 / CD138 antibody [B-A38]
|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, WB, Functional Assay|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
|IHC (Frozen sections)||1/100-1/500*
|IHC (Formalin-fixed paraffin-embedded sections)||1/100*
| Western blot||Assay-dependent dilution
| Functional Assay||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Functional Assay : This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by ion exchange chromatography|