TAFII68 antibody [N1N3]
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| Immunoprecipitation||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||62 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa|
|Product Description||Rabbit Polyclonal antibody to TAFII68 (TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa)|
|Background||Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. [provided by RefSeq]|
|Synonyms||Npl3 antibody, RBP56 antibody, TAF2N antibody, TAFII68 antibody, hTAFII68 antibody, TAF15 antibody, TAF(II)68 antibody, "TATA box binding protein (TBP)-associated factor, RNA polymerase II, N, 68kD (RNA-binding protein 56) antibody", 68 kDa TATA-binding protein-associated factor antibody, RBP56/CSMF fusion antibody, TATA-binding protein-associated factor 2N antibody, TATA box-binding protein-associated factor 2N (RNA-binding protein 56) antibody, TBP-associated factor 15 antibody, "TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human TAFII68. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Pig (91%)|