|Applications||ICC/IF, IHC-P, IP, WB, ChIP-seq|
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
| ChIP-sequencing||Assay-dependent dilution
Immunoprecipitation : target validated by Mass SpectrometryNot tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||45 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HeLa mock control , HeLa transfected shTDP-43 , Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||TAR DNA binding protein|
|Product Description||Rabbit Polyclonal antibody to TDP43|
|Background||HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]|
|Synonyms||ALS10 antibody, TDP-43 antibody, TARDBP antibody, TAR DNA-binding protein 43 antibody, TAR DNA-binding protein-43 antibody, TAR DNA binding protein antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human TARDBP. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Zebrafish (83%), Xenopus laevis (83%), Chicken (95%), Bovine (96%)|