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TET1 antibody [N3C1]

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207
Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207
Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

Cat. No. GTX124207

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Wm, IP, ChIP assay, IHC

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μl ($399)
PRODUCT

Summary

TET1 antibody recognizes TET1 protein, which is a methylcytosine dioxygenase that catalyzes the sequential steps of DNA demethylation. Expression of TET1 protein is spatiotemporally regulated during embryonic development. Abnormal TET1 activation is associated with cancer growth and metastasis.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

235 kDa. ( Note )

Positive Control

DDDDK-human TET1-transfected 293T , 293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TET1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11176491

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tet methylcytosine dioxygenase 1 , CXXC6 , LCX , bA119F7.1

Cellular Localization

Nucleus

Background

Dioxygenase that specifically binds methylcytosine (5mC), a minor base in mammalian DNA found in repetitive DNA elements that is crucial for retrotransposon silencing and mammalian development. Catalyzes the conversion of methylcytosine (5mC) to 5-hydroxymethylcytosine (hmC). The clear function of 5-hydroxymethylcytosine (hmC) is still unclear but it may influence chromatin structure and recruit specific factors or may constitute an intermediate component in cytosine demethylation. 5-hydroxymethylcytosine (hmC) is present in ES cells and is enriched in the brain, especially in Purkinje neurons. May play a role in the fetal development of heart, lung and brain.

Database

Research Area

DATA IMAGES

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207

GTX124207 ICC/IF Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:1000.
Blue: Hoechst 33342 staining.

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with TET1 antibody [N3C1] (GTX124207) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Hela xenograft, using TET1 (GTX124207) antibody at 1:1000 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124207

GTX124207 ICC/IF Image

TET1 antibody [N3C1] detects TET1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-TET1 antibody [N3C1] used in Western Blot (WB). GTX124207

GTX124207 WB Image

TET1 antibody [N3C1] detects TET1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of DDDDK-human TET1-transfected 293T cells
5% SDS-PAGE
TET1 antibody [N3C1] (GTX124207) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus on Human normal prostate tissue by immunohistochemical analysis.
Sample: Paraffin-embedded Human normal prostate tissue.
TET1 antibody [N3C1] (GTX124207) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
TET1 antibody [N3C1] (GTX124207) diluted at 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TET1 antibody [N3C1] used in IHC (Paraffin sections) (IHC-P). GTX124207

GTX124207 IHC-P Image

TET1 antibody [N3C1] detects TET1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
TET1 stained by TET1 antibody [N3C1] (GTX124207) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

TET1 antibody [N3C1] Cat. No. GTX124207

Rating ( Average 5 based on 9 users reviews)
Application
Western Blot(WB) ( Average 5 based on 5 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 2 users reviews)
ChIP assay(ChIP assay) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Endocrinol in 2020.PMID: 32089682
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Int J Endocrinol in 2020.PMID: 32089682
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Cancer in 2018.PMID: 30045709
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Clin Epigenetics in 2016.PMID: 26973719
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Clin Epigenetics in 2016.PMID: 26973719
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Clin Epigenetics in 2018. PMID: 30075814
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : ChIP assay
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2016. PMID: 27708396
Date : Anonymous submitted on 23-Dec-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Clin Epigenetics in 2018. PMID: 30075814
Date : Anonymous submitted on 14-Oct-2016
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human pancreatic ductal adenocarcinoma
Fixation : 10% paraformaldehyde
Primary Antibody : 1:200, 25°C, 2Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399