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TIM-1 antibody

KO/KD
Anti-TIM-1 antibody used in Western Blot (WB). GTX85068
Anti-TIM-1 antibody used in Western Blot (WB). GTX85068
Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85068
Anti-TIM-1 antibody used in Western Blot (WB). GTX85068
Anti-TIM-1 antibody used in Western Blot (WB). GTX85068
Anti-TIM-1 antibody used in Western Blot (WB). GTX85068
Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85068

Cat. No. GTX85068

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all TIM-1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/mL
IHC-P 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

3T3 (NIH) Cell Lysate(GTX27179) , Human Uterus Tissue Slide , Human Uterus Tissue Lysate

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TIM-1 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human TIM-1.The immunogen is located within amino acids 50 - 100 of TIM-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_10726144

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hepatitis A virus cellular receptor 1 , CD365 , HAVCR , HAVCR-1 , KIM-1 , KIM1 , TIM , TIM-1 , TIM1 , TIMD-1 , TIMD1

Cellular Localization

Membrane

Background

The human form of TIM-1 was initially discovered as a membrane glycoprotein through which the hepatitis A virus can gain entry into a cell. It was also identified as kidney injury molecule 1 (Kim-1), a predicted adhesion molecule that is upregulated on the surfaces of kidney epithelia. It is also expressed on T helper 2 (Th2) cells of the immune system, and following the binding of its natural ligand TIM-4, stimulates T cell expansion and cytokine production in response to viral challenge. It has been suggested that hyperactivation of TIM-1 leads to an increased level of Th2 responsiveness and asthma susceptibility, and antibodies to TIM-1 may therefore be a novel approach to treating asthma.

Database

Research Area

DATA IMAGES

Anti-TIM-1 antibody used in Western Blot (WB). GTX85068

GTX85068 WB Image

WB analysis of 3T3 cell lysate using GTX85068 TIM-1 antibody.
Working concentration : 1 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85068

GTX85068 WB Image

WB analysis of NIH/3T3 cell lysates using GTX85068 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 8 μg/ml

Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85068

GTX85068 IHC-P Image

IHC-P analysis of human uterus tissue using GTX85068 TIM-1 antibody.
Working concentration : 10 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85068

GTX85068 WB Image

WB analysis of various sample lysates using GTX85068 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 8 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85068

GTX85068 WB Image

KO/KD

WB analysis of HeLa cells were transfected with control siRNAs (lane 1) or TIM-1 siRNAs (lane 2) using GTX85068 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 8 μg/ml

Anti-TIM-1 antibody used in Western Blot (WB). GTX85068

GTX85068 WB Image

WB analysis of various sample lysates using GTX85068 TIM-1 antibody.
Loading : 15μg of lysates per lane
Dilution : 8 μg/ml

Anti-TIM-1 antibody used in IHC (Paraffin sections) (IHC-P). GTX85068

GTX85068 IHC-P Image

IHC-P analysis of human uterus tissue using GTX85068 TIM-1 antibody.
Working concentration : 20 μg/ml

REFERENCE

Application Reference

Karunasagara S et al. J Ethnopharmacol 2020; 254 : 112693 Korean red ginseng attenuates hyperglycemia-induced renal inflammation and fibrosis via accelerated autophagy and protects against diabetic kidney disease.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399