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TLR4 antibody

Anti-TLR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31675
Anti-TLR4 antibody used in Western Blot (WB). GTX31675
Anti-TLR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31675
Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31675
Anti-TLR4 antibody used in Western Blot (WB). GTX31675

Cat. No. GTX31675

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Rat
Package
100 μg ($399)
See all TLR4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 2 - 4 μg/mL
ICC/IF 2 μg/mL
IHC-P 2.5 μg/mL
FACS Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

K-562 Cell Slide(GTX25535) , K562 Cell Lysate(GTX27911) , Human Small Intestine Tissue Lysate(GTX27923)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

TLR4 antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of human TLR4.The immunogen is located within amino acids 30 - 80 of TLR4.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2755039

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

toll like receptor 4 , ARMD10 , CD284 , TLR-4 , TOLL

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This receptor has been implicated in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. Mutations in this gene have been associated with differences in LPS responsiveness. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-TLR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31675

GTX31675 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31675 TLR4 antibody.
Working concentration : 20 μg/ml

Anti-TLR4 antibody used in Western Blot (WB). GTX31675

GTX31675 WB Image

WB analysis of human small intestine tissue lysate using GTX31675 TLR4 antibody.
Working concentration : (A) 2 and (B) 4 μg/ml

Anti-TLR4 antibody used in IHC (Paraffin sections) (IHC-P). GTX31675

GTX31675 IHC-P Image

IHC-P analysis of human spleen tissue using GTX31675 TLR4 antibody.
Working concentration : 2.5 μg/ml

Anti-TLR4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX31675

GTX31675 ICC/IF Image

ICC/IF analysis of K562 cells using GTX31675 TLR4 antibody.
Working concentration : 2 μg/ml

Anti-TLR4 antibody used in Western Blot (WB). GTX31675

GTX31675 WB Image

The data was published in the journal PLoS Pathog in 2019. PMID: 31009511

REFERENCE

Application Reference

H Cui et al. Research Square 2023; TNF-α promotes osteocyte necroptosis by upregulating TLR4 in postmenopausal osteoporosis
Application : IHC-P
Qi L et al. J Cell Mol Med 2022; 26 (4) : 1264-1280 New insights into the central sympathetic hyperactivity post-myocardial infarction: Roles of METTL3-mediated m6 A methylation.
Application : WB
Reactivity : Rat
Chao CH et al. PLoS Pathog 2019; 15 (4) : e1007625 Dengue virus nonstructural protein 1 activates platelets via Toll-like receptor 4, leading to thrombocytopenia and hemorrhage.
Application : WB, FACS
Reactivity : Human
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399