|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, WB|
|Application Note||We recommend the following starting dilutions:
For WB: Use at a concentration of 1-3 ug/ml.
For ICC/IF: Use at a dilution of 1:10-1:2000.
For FACS: Use at a concentration of 2-5 ug/ 1x10^6 cells.
For IHC-Fr: Use at a dilution of 1:10-1:2000.
For IHC-P: Use at a assay dependent dilution.
Optimal dilutions should be determined experimentally by the end user.|
|Positive Controls||Human Ramos cells|
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||This antibody is affinity purified.|
|Full Name||toll-like receptor 7|
|Product Description||Rabbit polyclonal to TLR7|
|Background||The Toll like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL 1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin 1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1 10) have been described. Stimulation of the NFkB signaling pathway by TLR7 suggests that it plays a role in immune response.|
|Synonyms||TLR7, Q9NYK1, 300365, 51284|
|Cellular Localization||Type I membrane protein (By similarity).|
|Immunogen||Synthetic peptide: LSHNQLTTVPERLSNCSRSLKNL conjugated to KLH, corresponding to amino acids 706-728 of Human TLR7.|
|Species Reactivity||Human, Mouse, Rat|