TNF alpha antibody
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||26 kDa (note)
|Positive Controls||Raji , NCI-H929 , C8D30 , PC-12 , Rat-2 , Raw264.7 (50 ng/ml LPS treatment for 4 hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||tumor necrosis factor|
|Product Description||Rabbit Polyclonal antibody to TNF alpha (tumor necrosis factor)|
|Background||This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq]|
|Synonyms||DIF antibody, TNF-alpha antibody, TNFA antibody, TNFSF2 antibody, TNF antibody, TNF, monocyte-derived antibody, TNF, macrophage-derived antibody, APC1 protein antibody, cachectin antibody, tumor necrosis factor antibody, tumor necrosis factor-alpha antibody, tumor necrosis factor alpha antibody, TNF-a antibody, tumor necrosis factor ligand superfamily member 2 antibody, TNF superfamily, member 2 antibody|
|Cellular Localization||Cell membrane; Single-pass type II membrane protein , Tumor necrosis factor , soluble form: Secreted |
|Immunogen||Full length human TNF alpha Recombinant protein.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Cat, Pig, Rabbit, Rhesus Monkey, Chimpanzee, Bovine, Guinea pig|
|Predict Reactivity Note||Dog (90%), Cat (89%), Pig (86%), Rabbit (80%), Rhesus Monkey (96%), Chimpanzee (99%), Bovine (80%), Guinea pig (80%)|