TRAF6 antibody [N2C2], Internal
|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||60 kDa (note)
|Positive Controls||A549 , HeLa , HepG2 , NIH-3T3 , BCL-1 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||TNF receptor-associated factor 6|
|Product Description||Rabbit Polyclonal antibody to TRAF6 (TNF receptor-associated factor 6)|
|Background||The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. This protein mediates the signaling not only from the members of the TNF receptor superfamily, but also from the members of the Toll/IL-1 family. Signals from receptors such as CD40, TNFSF11/RANCE and IL-1 have been shown to be mediated by this protein. This protein also interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways. This protein functions as a signal transducer in the NF-kappaB pathway that activates IkappaB kinase (IKK) in response to proinflammatory cytokines. The interaction of this protein with UBE2N/UBC13, and UBE2V1/UEV1A, which are ubiquitin conjugating enzymes catalyzing the formation of polyubiquitin chains, has been found to be required for IKK activation by this protein. Two alternatively spliced transcript variants encoding identical proteins have been reported. [provided by RefSeq]|
|Synonyms||MGC:3310 antibody, RNF85 antibody, TRAF6 antibody, RING finger protein 85 antibody, TNF receptor-associated factor 6 antibody, E3 ubiquitin-protein ligase TRAF6 antibody, interleukin-1 signal transducer antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human TRAF6. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (90%), Rhesus Monkey (97%), Bovine (90%)|