|Applications||IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||438 kDa (note)
|Positive Controls||Jurkat , Raji , NCI-H929 , 293T , A431 , HeLa , HepG2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||transformation/transcription domain-associated protein|
|Product Description||Rabbit Polyclonal antibody to TRRAP (transformation/transcription domain-associated protein)|
|Background||Adapter protein, which is found in various multiprotein chromatin complexes with histone acetyltransferase activity (HAT), which gives a specific tag for epigenetic transcription activation. Component of the NuA4 histone acetyltransferase complex which is responsible for acetylation of nucleosomal histones H4 and H2A. Plays a central role in MYC transcription activation, and also participates in cell transformation by MYC. Required for p53/TP53-, E2F1- and E2F4-mediated transcription activation. Also involved in transcription activation mediated by the adenovirus E1A, a viral oncoprotein that deregulates transcription of key genes. Probably acts by linking transcription factors such as E1A, MYC or E2F1 to HAT complexes such as STAGA thereby allowing transcription activation. Probably not required in the steps following histone acetylation in processes of transcription activation. May be required for the mitotic checkpoint and normal cell cycle progression. |
|Synonyms||PAF400 Antibody, STAF40 Antibody, Tra1 Antibody, PAF350/400 Antibody, transformation/transcription domain-associated protein Antibody, TR-AP Antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human TRRAP. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Japanese medaka, Bovine|
|Predict Reactivity Note||Mouse (98%), Rat (98%), Japanese medaka (89%), Bovine (100%)|