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Tau (phospho Thr205) antibody

Orthogonal
Anti-Tau (phospho Thr205) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24841
Anti-Tau (phospho Thr205) antibody used in Western Blot (WB). GTX24841

Cat. No. GTX24841

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, IHC, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat, Dog
Package
50 μl ($399)
See all Tau products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF 1-2 μg/mL
IHC-P Assay dependent
FACS 3-5 μg/million cells
ELISA Assay dependent
IHC Assay dependent
IHC (Free Floating) Assay dependent
Not tested in other applications.

Calculated MW

79 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Tau that contains threonine 205. The sequence is conserved in mouse and rat.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_380444

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU

Cellular Localization

Cytoplasm, cytosol,Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Cell projection, dendrite

Background

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Tau (phospho Thr205) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24841

GTX24841 ICC/IF Image

ICC/IF analysis of SH-SY5Y cells using GTX24841 Tau (phospho Thr205) antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes
Dilution : 1 μg/ml in 1% BSA incubated for 3 hours at room temperature

Anti-Tau (phospho Thr205) antibody used in Western Blot (WB). GTX24841

GTX24841 WB Image

Orthogonal

WB analysis of samples using GTX24841 Tau (phospho Thr205) antibody. The data show that only the immunogen phosphopeptide blocks the signal, demonstrating the specificity of the antibody.

REFERENCE

Application Reference

Chen SM et al. Int J Mol Sci 2022; 23 (12) Microtube Array Membrane Encapsulated Cell Therapy: A Novel Platform Technology Solution for Treatment of Alzheimer's Disease.
Dejanovic B et al. Neuron 2018; 100 (6) : 1322-1336.e7 Changes in the Synaptic Proteome in Tauopathy and Rescue of Tau-Induced Synapse Loss by C1q Antibodies.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399