|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||23 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , Mouse liver , A-10 , A-10 treated with MG132 , *Bovine Smooth Muscle Cell , *Bovine Fibroblastic Chondrocyte|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Product Description||Rabbit Polyclonal antibody to Transgelin (transgelin)|
|Background||The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]|
|Synonyms||DKFZp686B01212 antibody, DKFZp686P11128 antibody, SM22 antibody, SMCC antibody, TAGLN1 antibody, WS3-10 antibody, TAGLN antibody, 22 kDa actin-binding protein antibody, smooth muscle protein 22-alpha antibody, transgelin variant 2 antibody, transgelin antibody, SM22-alpha antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat, Bovine|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Xenopus laevis, Pig, Chicken|
|Predict Reactivity Note||Xenopus laevis (81%), Pig (98%), Chicken (87%)|