Trident Nuclear Protein Extraction Kit
|Background||Trident Nuclear protein extraction kit is for fast extraction of native cytoplasmic and nuclear proteins from cultured mammalian cells or mammalian tissues. The procedure can be completed in less than 15 min. Extracted native cytoplasmic and nuclear proteins can be used for SDS-PAGE, immunoblottings, ELISA, IP, protein localization, gel mobility shift assays, 2-D gels etc.|
|Application Note||1. Read the entire protocol carefully. |
2. Protease inhibitors is not necessary prior to extraction. However if downstream application takes significant amounts of time or the protein extract will be stored for longer period of time, the addition of protease inhibitors to extracted lysate buffer is recommended.
3. The nuclear extraction buffer contains 300 mM salt, for some applications, dilution or desalting of the extract may be needed.
4. The capacity of protein extraction filter cartridge is 500 µL. Multiple filter cartridges can be used if larger amount of cell lysate is processed.
5. To study protein phosphorylation, phosphatase inhibitors should be added to lysis buffer prior to use.
|Storage Instruction||Store at 4°C|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|