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TrkA antibody

Anti-TrkA antibody used in Western Blot (WB). GTX54856
Anti-TrkA antibody used in Live cell imaging (LCI). GTX54856
Anti-TrkA antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54856
Anti-TrkA antibody used in IHC (Frozen sections) (IHC-Fr). GTX54856
Anti-TrkA antibody used in Flow cytometry (FACS). GTX54856

Cat. No. GTX54856

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr, FACS, IHC (Free Floating), LCI

Reactivity

Human, Mouse, Rat
Package
50 μl ($639)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IHC (Free Floating) Assay dependent
LCI Assay dependent
Not tested in other applications.

Calculated MW

88 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.025% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 (2nd  extracellular  immunoglobulin-like domain) of rat TrkA (Accession  : P35739).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

neurotrophic receptor tyrosine kinase 1 , Trk

Cellular Localization

Cell membrane

Background

a protooncogene; receptor that binds to nerve growth factor (NGF) [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-TrkA antibody used in Western Blot (WB). GTX54856

GTX54856 WB Image

WB analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain lysates using GTX54856 TrkA antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-TrkA antibody used in Live cell imaging (LCI). GTX54856

GTX54856 LCI Image

Live cell imaging analysis of live intact PC12 cells using GTX54856 TrkA antibody.
Panel A : Primary antibody (Red)
Panel B : Hoechst 33342 for nuclear staining (blue)
Panel C : Merged images of Panel A and B
Dilution : 1:50

Anti-TrkA antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX54856

GTX54856 IHC (Free Floating) Image

IHC-Frfl (free floating) analysis of rat hippocampal dentate gyrus tissue using GTX54856 TrkA antibody.
Panel A : Brain sections were stained using  Anti-TrkA  Antibody (green).
Panel B : The same section was also stained for glial fibrillary acidic protein (GFAP) (red and counterstained blue). 
Panel C : Overlay of A and B demonstrates co-localization of TrkA and GFAP in dentate gyrus astrocytes.
Dilution : 1:1000

Anti-TrkA antibody used in IHC (Frozen sections) (IHC-Fr). GTX54856

GTX54856 IHC-Fr Image

IHC-Fr analysis of rat DRG tissue using GTX54856 TrkA antibody. TrkA (red) is expressed in DRG neurons and in satellite microglia (arrows). Hoechst 33342 is used as the counterstain.
Dilution : 1:100

Anti-TrkA antibody used in Flow cytometry (FACS). GTX54856

GTX54856 FACS Image

FACS analysis of Jurkat cells using GTX54856 TrkA antibody.
Black : Unstained cell
Green : Cell staining with primary antibody
Dilution : 1:25

REFERENCE

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639