|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||28 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||U2 small nuclear RNA auxiliary factor 1|
|Product Description||Rabbit polyclonal antibody to U2AF35 (U2 small nuclear RNA auxiliary factor 1)|
|Background||This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq]|
|Synonyms||DKFZp313J1712 antibody, FP793 antibody, RN antibody, RNU2AF1 antibody, U2AF35 antibody, U2AFBP antibody, U2AF1 antibody, U2 snRNP auxiliary factor small subunit antibody, U2 small nuclear RNA auxillary factor 1 antibody, "U2 small nuclear ribonucleoprotein auxillary factor, 35-KD subunit antibody", U2(RNU2) small nuclear RNA auxiliary factor binding protein antibody, splicing factor U2AF 35 kDa subunit antibody, splicing factor U2AF 35kDa subunit antibody, U2 small nuclear RNA auxiliary factor 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human U2AF35. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Rat (99%), Xenopus laevis (97%), Chicken (98%), Bovine (98%)|