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1 .GTX114186 UBE2A antibody

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UBE2A antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
Cat No: GTX114186
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-UBE2A antibody used in Western Blot (WB). GTX114186
  • Anti-UBE2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114186
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  • Datasheet
  • Bio-info
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  • Application Information: UBE2A antibody

Catalog Number GTX114186
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Product Name UBE2A antibody
See all UBE2A products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size17 kDa (note) IsoForm (2)
Positive Controlsmouse brain
Form SuppliedLiquid
Concentration0.3 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: UBE2A antibody

Full Nameubiquitin-conjugating enzyme E2A (RAD6 homolog)
BackgroundThe modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Multiple alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms. [provided by RefSeq]
SynonymsHHR6A antibody, RAD6A antibody, UBC2 antibody, UBE2A antibody, ubiquitin carrier protein A antibody, RAD6 homolog A antibody, HR6A antibody, ubiquitin-conjugating enzyme E2 A antibody, ubiquitin-protein ligase A antibody, ubiquitin-conjugating enzyme E2A (RAD6 homolog) antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human UBE2A. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey
Predict Reactivity NoteRat (100%), Zebrafish (100%), Xenopus laevis (100%), Chicken (100%), Rhesus Monkey (100%)
ConjugationUnconjugated

  • Storage Conditions: UBE2A antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
UBE2A antibody validated data
  • Anti-UBE2A antibody used in Western Blot (WB). GTX114186
    • GTX114186 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX114186 diluted at 1:500
      Top
  • Anti-UBE2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114186
    • GTX114186 ICC/IF Image
      UBE2A antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: UBE2A protein stained by UBE2A antibody (GTX114186) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-UBE2A antibody used in Western Blot (WB). GTX114186
    • GTX114186 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse brain
      15% SDS PAGE
      GTX114186 diluted at 1:500
      Top
  • Anti-UBE2A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114186
    • GTX114186 ICC/IF Image
      UBE2A antibody detects UBE2A protein at cytoplasm and nucleus by immunofluorescent analysis.
      Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: UBE2A protein stained by UBE2A antibody (GTX114186) diluted at 1:500.
      Blue: Hoechst 33342 staining.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensUBE2A7319P49459, A6NGR2, A6NFE9312180BC010175AAH1017517.32
Homo sapiensUBE2A7319P49459, A6NGR2, A6NFE9312180NM_003336NP_00332717.32

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusUbe2a22209Q9Z255, Q3UCS1NM_019668NP_06264217.32
Mus musculusUbe2a22209Q9Z255, Q3UCS1NP_00130062516.98
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.