|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||17 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast)|
|Product Description||Rabbit Polyclonal antibody to UBE2D1 (ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast))|
|Background||The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. [provided by RefSeq]|
|Synonyms||E2(17)KB1 antibody, SFT antibody, UBC4/5 antibody, UBCH5 antibody, UBCH5A antibody, UBE2D1 antibody, ubiquitin-conjugating enzyme E2(17)KB 1 antibody, ubiquitin-conjugating enzyme E2-17 kDa 1 antibody, ubiquitin carrier protein D1 antibody, "ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast) antibody", UBC4/5 homolog antibody, ubiquitin-conjugating enzyme E2 D1 antibody, ubiquitin-protein ligase D1 antibody, stimulator of Fe transport antibody|
|Immunogen||Full length human UBE2D1 Recombinant protein.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (100%), Xenopus laevis (95%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)|