UBE2I antibody, N-term
|Applications||ICC/IF, IHC, IHC-P, WB|
|Application Note||Approx 17kDa band observed in Human Kidney, Human Lung and Human Testis lysates (calculated MW of 18kDa according to NP_003336). Recommended concentration: 0.1-0.3µg/ml In paraffin embedded Human Kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1µg/ml. This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX41193-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)|
|Product Description||Goat polyclonal antibody to UBE2I|
|Background||The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. Catalyzes the covalent attachment of ubiquitin-like protein SUMO-1 to other proteins. Forms a tight complex in association with two RAN GTPase interacting proteins, RANGAP1 and RANBP2. Interacts with E1A and E2A. Interacts with HIPK1 and HIPK2. Interacts with SIAH1 and PARP. Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
|Synonyms||ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9), ubiquitin-like protein SUMO-1 conjugating enzyme, ubiquitinconjugating enzyme E2I (homologous to yeast UBC9), ubiquitin conjugating enzyme 9, OTTHUMP00000198127, ubiquitinconjugating enzym, ubiquitin-conjugating enzym, ubiquitinlike protein SUMO1 conjugating enzyme, ubiquitin conjugating enzyme9, UBE2I, ubiquitin conjugating enzym|
|Specificity||This antibody is specific for UBE2I.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.