|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||18 kDa (note)
|Positive Controls||HeLa , HepG2 , Neuro2A , GL261 , C8D30 , mouse testis , whole zebrafish|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ubiquitin-conjugating enzyme E2L 3|
|Product Description||Rabbit Polyclonal antibody to UBE2L3 (ubiquitin-conjugating enzyme E2L 3)|
|Background||The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||E2-F1 antibody, L-UBC antibody, UBCH7 antibody, UbcM4 antibody, UBE2L3 antibody, ubiquitin-conjugating enzyme E2 L3 antibody, ubiquitin-protein ligase L3 antibody, ubiquitin carrier protein L3 antibody, ubiquitin-conjugating enzyme E2-F1 antibody, ubiquitin-conjugating enzyme UBCH7 antibody, ubiquitin-conjugating enzyme E2L 3 antibody|
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2L3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Zebrafish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (100%), Xenopus laevis (100%), Pig (100%), Chicken (100%), Rhesus Monkey (100%), Bovine (100%)|