UBE4B antibody [C2C3], C-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||146 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||ubiquitination factor E4B (UFD2 homolog, yeast)|
|Product Description||Rabbit Polyclonal antibody to UBE4B (ubiquitination factor E4B (UFD2 homolog, yeast))|
|Background||The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||E4 antibody, HDNB1 antibody, KIAA0684 antibody, UBOX3 antibody, UFD2 antibody, UFD2A antibody, UBE4B antibody, UFD2A-III/UBE4B-III splice isoform antibody, "ubiquitination factor E4B (UFD2 homolog, yeast) antibody", ubiquitination factor E4B (homologous to yeast UFD2) antibody, homozygously deleted in neuroblastoma 1 antibody, ubiquitin-fusion degradation protein 2 antibody, homozygously deleted in neuroblastoma-1 antibody, ubiquitin fusion degradation protein 2 antibody, ubiquitin conjugation factor E4 B antibody, homologous to yeast UFD2 antibody|
|Cellular Localization||Cytoplasm |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Zebrafish, Bovine|
|Predict Reactivity Note||Mouse (99%), Rat (98%), Zebrafish (90%), Bovine (100%)|