|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||33 kDa (note)
|Positive Controls||293T , A431 , HeLa , NIH-3T3 , JC , BCL-1|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||UBX domain protein 1|
|Product Description||Rabbit Polyclonal antibody to UBXN1 (UBX domain-containing protein 1)|
|Background||Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol. |
|Synonyms||2B28 antibody, SAKS1 antibody, UBXD10 antibody, UBXN1 antibody, SAPK substrate protein 1 antibody, UBX domain-containing protein 1 antibody, UBA/UBX 33.3 kDa protein antibody, UBX domain protein 1 antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human UBXN1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Bovine (84%)|