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UEA I Lectin (FITC)

Cat. No. GTX01512

Reference 9

Application

 ICC/IF

Species

 Ulex europaeus
Package
500 μg ($149)
See all UEA I Lectin products
PRODUCT

Summary

The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column.

APPLICATION

Product Note

Fucose blood group H

PROPERTIES

Form

Liquid

Buffer

10mM Phosphate (pH7.6), 150mM NaCl, 0.1mM Calcium Chloride

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Region/Sequence

Native Protein

Expression System

Native Protein

Purification


Purified from Ulex europaeus

Conjugation

Fluorescein isothiocyanate (FITC)
Absorption : 490 nm ; Emission : 520 nm. Ratio : 1.0-1.4 molecules FITC (isomer 1) per Lectin molecule.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UAE , Ulex europaeus , UAE I Lectin , Fucose lectin , UAE I , Ulex europaeus lectin , UAE Lectin

Background

The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R

Research Area

REFERENCE

Application Reference

Edwards MM et al. Invest Ophthalmol Vis Sci 2023; 64 (3) : 2 Clinicopathologic Findings in Three Siblings With Geographic Atrophy.
Boger-May A et al. Gut Microbes 2022; 14 (1) : 2123677 Altered microbial biogeography in an innate model of colitis.
Ruan Z et al. Foods 2022; 11 (16) Si-Wu Water Extracts Protect against Colonic Mucus Barrier Damage by Regulating Muc2 Mucin Expression in Mice Fed a High-Fat Diet.
Zehan Hu et al. Science 2021; 374 (6568) : eabe6723 Small proline-rich protein 2A is a gut bactericidal protein deployed during helminth infection.
Lee YN et al. Sci Rep 2021; 11 (1) : 13449 Ultrasonic microbubble VEGF gene delivery improves angiogenesis of senescent endothelial progenitor cells.
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 149