| Catalog Number | GTX01510 |
| Product Name | UEA I Lectin |
| Full Name | Ulex europaeus Lectin |
| Synonyms | | UAE | Ulex europaeus | UAE I Lectin | Fucose lectin | UAE I | Ulex europaeus lectin | UAE Lectin | |
| Product Description | Ulex europaeus lectin against fucose blood group H |
| Background | The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.
Inhibiting/Eluting sugars: 50-100 mM L-fucose
Carbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R
|
| Specificity | Fucose blood group H |
| Applications | Conjugation, Purification. The usefulness of this product in other applications has not been determined. |
| Application Note | Conjugation to Sepharose 4B (solid phase columns) to purify blood group glycoproteins. For conjugation azide should be removed. Dilute in buffer containing 0.1 mM calcium chloride. The optimum dilution should be determined by the individual lab. |
| Target | Fucose blood group H |
| Form Supplied | Liquid |
| Concentration | 2 mg/ml |
| Storage Buffer | 10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide |
| Storage Instruction | Store at 2-8°C. |
| Notes | For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. |