ULK2 antibody [C2C3], C-term

Cat. No. GTX111476

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all ULK2 products

View product citations for antibody GTX111476 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

NT2D1 , Neuro2A , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ULK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-51 like autophagy activating kinase 2 , ATG1B , Unc51.2

Background

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Gene ID: 9706 ULK2
UniProt: Q8IYT8 ULK2

Research Area

DATA IMAGES

GTX111476 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111476 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111476 WB Image

ULK2 antibody [C2C3], C-term detects ULK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX111476 ICC/IF Image

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

GTX111476 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ULK2(GTX111476) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX111476 WB Image

GTX111476 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ULK2(GTX111476) antibody at 1:200 dilution.

GTX111476 IHC-P Image

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min