UNKL antibody [N1N2], N-term

Cat No:

GTX120163

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Catalog Number

GTX120163

Product Name

UNKL antibody [N1N2], N-term

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Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

74 kDa (note) IsoForm

Positive Controls

HeLa

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Full Name	unkempt homolog (Drosophila)-like
Background	May participate in a protein complex showing an E3 ligase activity regulated by RAC1. Ubiquitination is directed towards itself and possibly other substrates, such as SMARCD2/BAF60b. Intrinsic E3 ligase activity has not been proven.
Synonyms	C16orf28 antibody, FLJ12623 antibody, FLJ23360 antibody, KIAA0734 antibody, MGC5179 antibody, ZC3H5L antibody, ZC3HDC5L antibody, UNKL antibody, putative E3 ubiquitin-protein ligase UNKL antibody, RING finger protein unkempt-like antibody, unkempt-like antibody, unkempt homolog (Drosophila)-like antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human UNKL. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Mouse
Predict Reactivity Note	Mouse (94%)
Conjugation	Unconjugated

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

UNKL antibody [N1N2], N-term validated data

GTX120163 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UNKL(GTX120163) antibody at 1:200 dilution.

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GTX120163 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UNKL(GTX120163) antibody at 1:200 dilution.

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Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	UNKL	64718	Q96EV1, Q9H9P5		BC011924	AAH11924	31.8
Homo sapiens	UNKL	64718	Q96EV1, Q9H9P5		NM_001037125	NP_001032202	31.76
Homo sapiens	UNKL	64718	Q96EV1, Q9H9P5		NM_001193388	NP_001180317	74.25