USP20 antibody, C-term
|Applications||IHC, IHC-P, WB|
|Application Note||Preliminary experiments gave no signal but low background in Human Liver extracts at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? In paraffin embedded Human Lymph Node shows strongest staining in smooth muscle cells surrounding a vessel. Recommended concentration, 3 µg/ml. All reported variants (NP_006667.3; NP_001103773.2; NP_001008563.2) encode the same protein.|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX25272-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||ubiquitin specific peptidase 20|
|Product Description||Goat Polyclonal antibody to USP20|
|Background||Deubiquitinating enzyme involved in beta-2 adrenergic receptor (ADRB2) recycling. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor (ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains. |
|Synonyms||ubiquitin specific protease 20, pVHL-interacting deubiquitinating enzyme 2, LSFR3A, VDU2, ubiquitin specific protease20, ubiquitin specific peptidase20, USP20, ubiquitin specific peptidase 20, USP20 / VDU2, pVHLinteracting deubiquitinating enzyme2, KIAA1003, pVHLinteracting deubiquitinating enzyme 2|
|Cellular Localization||According to PubMed:12865408, it localizes in the endoplasmic reticulum; however the relevance of such result is unclear. |
|Specificity||This antibody is specific for USP20 / VDU2.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog, Pig, Bovine|