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1 .GTX106253 USP47 antibody

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USP47 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
  • Publication  ( 1 )
Cat No: GTX106253
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
  • Anti-USP47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106253
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    Reference (1)
  • Bio-info
  • Protocol & MSDS

  • Application Information: USP47 antibody

Catalog Number GTX106253
☆☆☆☆☆
( 0 )
Publication ( 1 )
Product Name USP47 antibody
See all USP47 products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size157 kDa (note) IsoForm (3)
Positive ControlsA549 , HeLa , HepG2 , HCT116 , mouse brain
Form SuppliedLiquid
Concentration0.82 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: USP47 antibody

Full Nameubiquitin specific peptidase 47
BackgroundPutative ubiquitin-specific-processing protease that regulates cell growth and survival. Probably regulates CDC25A expression at a transcriptional level. May be catalytically inactive although it seems to have kept all necessary active site residues.
SynonymsDKFZp686C13257 antibody, FLJ20727 antibody, TRFP antibody, USP47 antibody, ubiquitin carboxyl-terminal hydrolase 47 antibody, ubiquitin-specific-processing protease 47 antibody, Trf (TATA binding protein-related factor)-proximal homolog antibody, ubiquitin specific protease 47 antibody, deubiquitinating enzyme 47 antibody, ubiquitin thiolesterase 47 antibody, ubiquitin thioesterase 47 antibody, ubiquitin specific peptidase 47 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human USP47. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Rat, Bovine
Predict Reactivity NoteRat (92%), Bovine (93%)
ConjugationUnconjugated

  • Storage Conditions: USP47 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
USP47 antibody validated data
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
    • GTX106253 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      5% SDS PAGE
      GTX106253 diluted at 1:1000
      Top
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
    • GTX106253 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      5% SDS PAGE
      USP47 antibody
      GTX106253 diluted at 1:1000
      Top
  • Anti-USP47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106253
    • GTX106253 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using USP47(GTX106253) antibody at 1:200 dilution.
      Top
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
    • GTX106253 WB Image
      Sample (50 ug of whole cell lysate)
      A: Mouse brain
      5% SDS PAGE
      GTX106253 diluted at 1:1000
      Top
  • Anti-USP47 antibody used in Western Blot (WB). GTX106253
    • GTX106253 WB Image
      Sample (30 ug of whole cell lysate)
      A: Hela
      5% SDS PAGE
      USP47 antibody
      GTX106253 diluted at 1:1000
      Top
  • Anti-USP47 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX106253
    • GTX106253 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using USP47(GTX106253) antibody at 1:200 dilution.
      Top
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  • Reference for USP47 antibody
Application References Application Reactivity
Wu C et al. USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization. Cell Death Differ (Epub ahead of print) (2018). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensUSP4755031Q96K76NP_001269588154.72
Homo sapiensUSP4755031Q96K76NP_001317137157.34
Homo sapiensUSP4755031Q96K76NM_017944NP_060414147.25

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusUsp4774996Q8BY87NM_133758NP_598519154.87
Mus musculusUsp4774996Q8BY87NM_177249NP_796223157.48
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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All products are for research use only—Not for use in diagnostic procedures.