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Ubiquitin antibody

Orthogonal
Anti-Ubiquitin antibody used in Western Blot (WB). GTX19247
Anti-Ubiquitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19247
Anti-Ubiquitin antibody used in Western Blot (WB). GTX19247

Cat. No. GTX19247

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC

Reactivity

 Species independent
Package
100 μl ($399)
See all Ubiquitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-5μg/ml
ICC/IF Assay dependent
IHC 1-10μg/ml
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Product Note

This antibody recognizes free ubiquitin as well as ubiquinated proteins in immunoblots.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.2, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Native bovine ubiquitin conjugated to KLH.

Purification

Protein A purified

Conjugation

Unconjugated

RRID

AB_425189

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-Ubiquitin antibody used in Western Blot (WB). GTX19247

GTX19247 WB Image

WB analysis of human brain cortex (senile plaque) tissue lysate using GTX19247 Ubiquitin antibody.

Anti-Ubiquitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX19247

GTX19247 ICC/IF Image

Orthogonal

The data was published in the journal Front Oncol in 2019. PMID: 31024851

Anti-Ubiquitin antibody used in Western Blot (WB). GTX19247

GTX19247 WB Image

The data was published in the journal Front Oncol in 2019. PMID: 31024851

REFERENCE

Application Reference

Lee M et al. J Cell Biol 2020; 219 (2) Ecm29-mediated proteasomal distribution modulates excitatory GABA responses in the developing brain.
Huang FI et al. Front Oncol 2019; 9 : 249 MPT0G413, A Novel HDAC6-Selective Inhibitor, and Bortezomib Synergistically Exert Anti-tumor Activity in Multiple Myeloma Cells.
Tung YT et al. Cell Rep 2015; 11 (8) : 1305-18 Mir-17-92 Governs Motor Neuron Subtype Survival by Mediating Nuclear PTEN.
Application : WB
Hsu HY et al. Carcinogenesis 2013; 34 (4) : 874-84 Fucoidan induces changes in the epithelial to mesenchymal transition and decreases metastasis by enhancing ubiquitin-dependent TGFβ receptor degradation in breast cancer.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399