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Ubiquitin antibody

Anti-Ubiquitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX51517
Anti-Ubiquitin antibody used in Western Blot (WB). GTX51517
Anti-Ubiquitin antibody used in IHC (Paraffin sections) (IHC-P). GTX51517

Cat. No. GTX51517

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Species independent
Package
100 μl ($399)
See all Ubiquitin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:300-1000
ICC/IF 1:50-200
IHC-P 1:50-400
Not tested in other applications.

Calculated MW

77 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

1% BSA, 50% Glycerol

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide derived from human Ubiquitin(5-55).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin C , HMG20

Cellular Localization

Nucleus

Background

This gene represents a ubiquitin gene, ubiquitin C. The encoded protein is a polyubiquitin precursor. Conjugation of ubiquitin monomers or polymers can lead to various effects within a cell, depending on the residues to which ubiquitin is conjugated. Ubiquitination has been associated with protein degradation, DNA repair, cell cycle regulation, kinase modification, endocytosis, and regulation of other cell signaling pathways. [provided by RefSeq, Aug 2010]

Database

Research Area

DATA IMAGES

Anti-Ubiquitin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX51517

GTX51517 ICC/IF Image

ICC/IF analysis of PC3 cells using GTX51517 Ubiquitin antibody.
Green : Primary antibody
Blue : DAPI
Dilution : 1:50

Anti-Ubiquitin antibody used in Western Blot (WB). GTX51517

GTX51517 WB Image

WB analysis of Ubiquitin protein lysate using GTX51517 Ubiquitin antibody.
Dilution : 1:300

Anti-Ubiquitin antibody used in IHC (Paraffin sections) (IHC-P). GTX51517

GTX51517 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX51517 Ubiquitin antibody.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399